Effect of microbial phytase and Nigella sativa seed extract supplementation on some hematological parameter in albino rats treated to cadmium chloride toxicity

Abstract

The present study was conducted to determine the role of microbial phytase and Nigella sativa seed alcoholic extract in relieving the toxicological effects of cadmium chloride in some hematological parameters (RBC count, Hb concentration, PCV, total WBC count, Differential WBC count, and concentration of ALT, AST, & ALP). Fourty mature male rats were randomly divided into 4 equal groups (control and three treated groups). All rats were drenched CdCl222 solution (30 mg/L).T1 supplemented with the microbial phytase (500 mg/kg of provender), T2 drenched the black seed extract (200 mg/L). and T3 received both of treatments. Eight weeks later, blood samples were obtained by heart puncture for estimation of the hematological parameters included in the present study. The results revealed a significant increase (P<0.05) in RBC count, Hb concentration and PCV of T1, T2 and T3 groups compared with control especially in T3, while there is no significant differences (P>0.05) in total WBC count. Neutrophils & eosinophils numbers showed a significant decrease in T1, T2 & T3 groups when compared with control, while lymphocytes showed an opposite results. ALT, AST, & ALP concentrations in T1, T2 & T3 groups showed a significant decrease (P<0.05) in comparison with control, especially that of T3.
It can be concluded that the microbial phytase supplementation and Nigella sativa seed alcoholic extract drenching have an efficient role in relieving the toxicological effects of CdCl222 in rats especially when supplemented with each other.