Isolation and identification of native species of Trichoderma in Wasit Province and evaluate the affectivity of them against Meloidogyne javanica

Abstract

The study had been conducted to detect of Trichoderma spp in different locations in Wasit province and evaluate the effect of them against Meloidogyne javanica in laboratory and green houses. Seven isolates belong to four species were isolated from Wasit province which were T .harzianum , T.hamatum , Trichoderma sp1 and Trichoderma sp2 .The results revealed that all tested isolates have high efficiency in parasitize eggs of nematode and the most effective was T. harzianum 3 in percent of parasitism 97% and with significant difference compared with the other isolates . The result also showed that there is clear effect of fungal filtrate in three concentrations (100, 50, and 25%) in mortality of eggs and the more effective filtrate is the filtrate of T. harzianum 3 and the percentages of mortality were 96.7, 75.5, 56.3% at the concentrations 100, 50, 25% respectively. The isolate T. harzianum 3 exist high efficiency in controlling nematode and promote plant growth with increasing the length of shoot and roots which were 19.7, 21.3cm respectively compared with the control 16.1, 15.4cm respectively. This isolate was also the most effective one in increasing wet and dry weight of shoot and roots system with significant superiority of other treatments.