Efficacy of some local isolates of Beauveria bassiana (Bals) Vuill and Metarhizium anisopliae (Met.) in controlling of cucurbit fly Dacus ciliates Loew

Abstract

The study has been conducted to evaluate the effect of four native fungal isolates on cucurbit fly D. ciliatus , two of these belong to Metarhizium anisopliae Met and two belong to Beauvveria bassiana Bals. The results revealed that the different concentrations of condial suspension of tested isolates have different effect on the adults and the concentration 109 spore / ml showed higher percent of mortality in lower period. The percentage of survival of females that treated with concentrations 105 , 107 , 109 spore/ml , were 96 , 80 , 32% respectively after two days of treatment with the isolate M. anisopliae ( MARD106) while the percentage of males were 100 , 86 and 28% respectively . The result of LC50 study revealed that the isolate B.bassiana (MARD66) was the most effective isolate and the LC50 was 105 spore / ml for males and females. The result also revealed that the study of the effect of female age on the isolates showed that the percentage of mortality was 95% in the case of one day old female treated with the isolate B. bassiana ( MARD66 ) while the percentages were 55 , 7.5% in the case of 4 , 7 days old female respectively. There is a significant differences in the susceptibility of female in different age when treated with the isolate and the percentage of mortality was 95% in one day old female treated with the isolate B. bassiana ( MARD66 ) while the percentages were 55 , 7.5% in 4 , 7 days old female respectively. All isolates recorded high percentages of mortality in the last instar larvae of insect and prevent this larval stage from develop to diapose stage.