Hematological, biochemical and histopathological changes in the rabbits treated with prednisone and olive oil

Abstract

Aim: the purpose of this study was to investigate the effect of olive oil on the serological and histological changes in rabbits after treatment. And compared the effect of olive oil with of prednisolone.
Materials and Methods: Twenty male rabbits were randomly divided into four groups. Grop-1 control group C. (n = 5) received Distilled water 2 ml /kg b.w./ day / orally and intramuscularly (i.m) injection of normal saline 2 ml /kg b.w./ day.
Group-2 first treated T1. (n=5) received Distilled water 2ml/kg /day / orally and injected prednisolone 2 mg/kg/day/ i.m .
Group-3 second treated T2. (n=5) received 2ml /kg/day /orally Olive oil and i.m. injected of normal saline 2 ml/kg/day.
Group-4 third treated T3. (n=5) received olive oil 2ml/kg/day/orally and prednisolone 2mg /kg/day/i.m .
After 14 days, blood samples were obtained for Red Blood Cell count (RBCc); Hemoglobin (Hb); Pack cell volume (pcv) and serological evaluation of serum lipids.
Animal were sacrificed and samples from Heart, lung and small intestine (Ileum) were obtained for Histopathological study.
Results: olive oil and prednisolone with 2mg\kg\day for fourteen days induce significant changes in the RBCc, Hb and serum lipids of the rabbits. While PCV% not induce any change.
Histopathological examination scores were significantly in T1 compared to other groups.
Conclusion: olive oil if effective in reducing damage in prednisolone – Treated rabbits.