Reducing genotoxicity of cyclophosphamide by hydro-coholic leaves extract of allium porrum in normal mouse bone marrow stem cells

Abstract

This paper evaluates percentage of chromosomal aberration (CA )and mitotic index (MI) in mice bone marrow as a method for assessing the ability of hydro-ethanolic leaves extract of leek ( Allium Porrum) to reduce genotoxicity of Cyclophosphamide (CP) . Mice were allocated in to 4 groups, two negative controls ,the first group receive ( 0.2 ml) D.W. and the second group was treated with (0.1ml) DMSO, the positive control group, (CP) 40mg/kg was given single intraperitoneally dose for 24 hr , while the treatment groups were given different doses of extract (75, 150,200and 250 mg/kg ) for 5 consecutive days and the same dose of CP . Experimental data was analyzed using (one –way ANOVA). The results demonstrated the intermediate dose (150 mg/kg) of extract increased in MI significantly and reduced CA (P<0.01) significantly .This study concluded that Allium porrum leaves were able to reduce genotoxicity of CP and protect normal proliferating cells in bone marrow from the effect of CP.