Immune response study of salmonella mbandaka isolated from human in (balb/c) mice

Abstract

The bases of this study is to evaluate the immune response to experimental infection of (BALB/c) mice by Salmonella Mbandaka . The experiment was carried out on one hundred mice of both genders with age range (6 – 8) weeks old, the mice were divided randomly into two groups (group A: includes 50 mice were given orally on infective dose (1.3×107C.F.U\ml) ,group B: includes 50 mice which inoculated orally with 1 ml of phosphate buffer saline (pH=7.2) and considers as control group).The study has showed that the experimentally infected mice (group A) are able to induce humoral and cellular immune response . The humoral immune response was detected by Enzyme-linked Immunosorbent Assay post induced infection in mice with infected dose. Maximum titers of ( IgG ) antibodies reached of (211.13) at four weeks , slight reduction at six weeks , reached a mean (179.41 ± 29.47), while in the eight weeks after infection, there was a reduction in the(IgG) titers to reach a mean (58.83 ± 34.56). While group B showed the normal range of IgG titer (20.22 ± 1.11 , 20.79 ±1.71 , 19.81 ± 0.88, 20.22 ± 1.11 , 20.97 ± 0.57 ) at zero time , 2 ,4 , 6 ,8 weeks respectively . The cellular immune response was detected by using the skin test mean thickness of the right foot pad was (2.85±0.06) millimeter after 24 hours, then increased to reach (3.69 ±0.07) mm after 48 hours , then declined to reach (2.71±0.09)mm after 72 hours in mice infected by infective dose. This study concluded that Salmonella Mbandaka infection of the host was able to induce both humoral and cellular immune responses, and these responses are dose dependent.