Analysis of the Pharmacological and Phytochemical constituents of the phenolic extracts of Hibiscus sabdariffa L. dried calyces plant by HPLC Techniques

Abstract

The chemical constituents of the secondary metabolic products of plants have important role in the discovery of therapeutically effective compounds. Hibiscus sabdariffa L. is widely utilized in herbal medicine for treatment and prevention of several diseases, the objective of this study is the Quantitative and Qualitative analysis of the phenolic extracts of the plants. High Performance Liquid Chromatography Techniques (HPLC) were used in the study, the result showed that the phenolic extracts of the plant composed of crud phenols ( 16%), crud alkaloid (8%), and crud terpens (5%). The HPLC identification of the extract indicates the presence of ( 7 ) compounds for phenolic extracts ; Vitamin C (443.39 μg/ml), Gossypetrin (289.52 μg/ml), Sabdaretin(222.61 μg/ml), Gossypetin (712.24 μg/ml ), Chlorogenic acid (657.22 μg/ml), Coumaric A (432.22 μg/ml ), and Hebiscetin (336.46 μg/ml). The highest phenolic compounds concentration was Gossypetin (712.24 μg/ml), while the lowest was Sabdaretin (222.61 μg/ml). The conclusion is that, the phenolic extract of the plant has many phytochemical and pharmacological components.