Detection of Virulence Gene ToxA in Pseudomonas aeruginosa Isolated From Otitis Infection

Abstract

One hundred fifty swabs were collected from otitis infection to detect toxA gene in Pseudomonas aeruginosa. These samples were cultured on MacConkey agar and blood agar media under sterilization condition and incubated for 18-24 hrs at 37oC. Morphological and biochemical test was carried out to identify P. aeruginosa in these samples , then diagnosis was confirmed with VITEK- 2 Compact system. In all these samples found twenty seven isolates belong to P. aeruginosa (18%).Antibiotic susceptibility test was achieved by select five antibiotics (Ticarcillin 75 μg,Ceftazidime 30μg,Cefotaxime 30μg,Meropenem 10μg,Levofloxacin 5μg) from different groups, all these twenty seven isolates were examined against these antibiotics. The results showed ability of these isolates to resist most antibiotics. Some of these isolates (Q1, Q2, Q5, Q8 and Q11)were resistant against all antibiotics. These five multidrug resistance isolates were selected to detecttox A gene. After extraction of genomeof all these five isolates by specific kit and amplificationoftox A gene by specific primers, all these five isolates were contained toxA gene in about 535 bp in its genetic materials.