Isolation and identification of Pasteurella multocida from healthy and clinical cases of sheep and study their antimicrobial sensitivity

Abstract

The study was entirely conducted in Basrah province which commenced from early of December 2017 until April 2018. Totally, seventy five samples were collected from the nasal passage of sheep’s that randomly selected from the farms and from the lung and liver of slaughtered sheep at the Al-Qurnah abattoir. Five antimicrobial susceptibility testing were performed in order to identify P. multocida. Twenty one isolates (28%) of samples were identified as P. multocida through using selective media (Clindamycin Gentamicin medium). The morphological characterization identification of these bacteria was achieved using additional biochemical tests of Api20NE system. The total isolation was 33.3% from apparently healthy sheep and (40%) from the infected lungs. The frequency of bacterial isolates was found to be slightly greater in female sheep (38%) than in male (29.1%). All isolates were observed to be resistant for three antibiotics comprised ampicillin, bacitracin, and penicillin.