Molecular Study to Detect MRSA Strains in Different Clinical Cases

Abstract

Staphylococcus aureus is an opportunist that causes systemic infections in the human being body. This organism increases its resistance to many categories of antibiotics more resistant.  Given this fact, a total of one hundred seventy-two (N = 172) samples, and the total number of positive samples for Staphylococcus aureus was seventy-seven (N = 77) samples. The number of positive which included 30 (38.39 %) pus samples, 27 (35.0 %) urine samples, 11 (14.28 %) of nasal swabs, and 9 (11.68 %) blood samples. These isolates were identified according to culture, microscopic examination, biochemical tests, and APIstaph system identification kits. The results showed 34 (44.15 %) of isolated S. aureus were MRSA strain. regarding of the molecular identification of MRSA the results showed mecA and Insertion sequence (IS431) is present in all cases in percent 100 % (34/34), (IS127) were 100 %, (34/34). Other gens were   gene of ccrC 20/34 (57.14 %) and ccrA2-B gene 6/34 (17.64 %).