To isolate and diagnose the bacteria Bacillus thuringiensis of some soils Wasit Province and use it as bio-pesticide with different concentrations to kill Agrotis ipsilon ( Hufnagel) laboratory

Abstract

The Bacillus thuringiensis found in all the study zones in Wasit province, but at different rates because difference type the land and climatic situation in each region. The average of bacteria in those areas 30.0،Swairah and Badra recorded higher rates for this bacteria 50% and 40% respectively. The Bacillus thuringiensis in soils Districts Al-ahrar and Al-hai was least 20% and 10% respectively .The study indicated to three of Bacillus thuringiensis dilutions differed in kill the larvae of the worm black biting Agrotis ipsilon laboratory, The B .thuringiensis dilution (57 × 106) Spor / ml killed higher of other dilutions and was 77.5% after 3 days of treatment of killing rate decreased with increasing the length of time .The dilution B .thuringiensis (57 × 106) Spor / ml recorded 30.7% kill rate during the treatment . dilution B .thuringiensis (34 × 105) Spor / ml least in kill of larvae of the worm black biting Agrotis ipsilon laboratory and was 59.2% after 3 days of treatment in the dilution .The kill rate decreased with increasing the time .Recorded general average for ratio killing of the dilution 25.0% during the time of the treatment.