Multi drug resistance in colistin resistant pseudomonas aeruginosa isolates and detection of MexXY efflux pump gene

Abstract

One Hundred and Twenty isolates of Pseudomonas aeruginosa were collected from different samples during the period August /2014 to December /2010 .Sensitivity of isolates was tested against colistin , results revealed that 27 isolates(22.5%) were resistance to colistin. The colistin resistant isolates ( and 21 sensitive isolates) were tested against 12 different antibiotics ,the results showed that all isolates were resistance to amoxicillin and carpencillin .Norfloxacin , Levofloxacin and Ciprofloxacin were found to be the most effective agents against the isolates. On the other hand the results showed that all isolates were able to produce hemolysin and biofilm formation. Polymerase Chain Reaction (PCR) technique was performed using specific primer targeting the specific sequences of the mexY gene, The results showed that mexY gene found in 74% of colistin resistant isolates.