Detection of human Metapneumovirus and respiratory Syncytial Virus associated with asthmatic patients using direct fluorescent assay and Real time – PCR

Abstract

This study aimed to determine the incidence of human respiratory syncytial virus (hRSV) and metapneumovirus (hMPV) occurrence in asthmatic patients in Wasit province. Blood samples were collected for measure the total and differential white blood cells count (WBCs). Correlation results of total and differential WBCs count for neutrophils, lymphocytes, eosinophils and basophils count among studied groups were significant (P <0.001). Enzyme linked immunosorbent assay (ELISA) technique has been applied for detection of total-IgE antibodies. Results revealed that the highest total-IgE antibodies titer in sera were significantly difference (p < 0.01). A total of 80 nasopharyngeal swabs were immediately dipped in transport media and stored until using for the detection of suspected hRSV and hMPV patients group by direct fluorescent assay the results appeared 13 (16.25 %) and 11 (13.75 %) samples were given positive results for hRSV and hMPV, respectively. Results of hRSV in asthmatic patients were subjected to real time – polymerase chain reaction (RT-PCR) appeared 15 samples out of 80 samples (18.75 %) were gave positive result for this test.