Valuating of sister chromatid exchange (SCE) in patients with type 2 diabetes mellitus

Abstract

This study was established to test the cytogenetic parameter of sister chromatid exchange (SCE) in type 2 diabetic patients for its close relationship with mutations. In this study, cytogenetic analyses were done on peripheral blood lymphocytes obtained from 25 type 2 DM patients using short-term lymphocyte culture .Human lymphocytes have long been used to assess the gene damage occurring in vivo as well as in vitro. Results showed significant high frequencies of SCEs were scored in type 2 DM patients [2.25±0.08SCE/cell] compared with control [1.28±0.04SCE/cell] (P<0.01). We concluded that the DM patients have significantly more genetic damage (high SCE frequency), and there are no significant differences in diabetic patients in relation with demographic characteristics (age and gender).