PCR conventional for detecting AP and PLA virulence Use factors of Entamoeba histolytica in patients stool samples in Al-Qadisiyah Province

Abstract

The present study designed to diagnose the Entamoeba histolytica parasite from patients with diarrhea attended to Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Qadisiya Province, Theirs ages less than one year to 14 years at the period from the beginning of May to the end of November 2014 . The number of samples that have been collected (100) stool samples, and the number of samples infected is (36) sample ; After samples were examined in direct wet smear method using normal saline, include the study to investigate and to emphasize the presence of virulence factors amoebapore and phospholipase in parasite using Conventional PCR Technique.
The result showed that the infection rate was 36% (100). The age group of l-5 years showed the highest rate of infection (41.6%), while patients aged11-14 years showed the lowest rate of infection (11.1%). The infection rate in male was higher (58.3%) than in female (41.6%). It was found that the majority of cases (61.1%) were from Urban areas.
DNA was extracted from positive from stool samples and then after amplified using special designed for primers E. histolytica virulence factors genes that called amoebapore &phospholipase and the amplified DNA passed in Electrophoresis apparatus for DNA. The results showed that the mentioned factor is present in all positive samples of E. histolytica.