Sub-chronic Effects of Zinc oxide Nanoparticles on Ovary, Gills, Kidney and Liver of Fresh Water Fish (Cyprinus carpio): histopathological Study

Abstract

Concerns have been raised about the potential environmental dangers connected with ZnO nanoparticles (ZnO NPs) entering aquatic systems due to the growing use of ZnO NPs in various fields. This study looked about how freshwater fish (Cyprinus carpio) respond to sub-chronic exposure to ZnO NPs through food. Common carp (C. carpio) from nearby fish hatcheries were employed in this investigation (city of Madaan, south of Baghdad). These fish had an approximate length and weight of 15-18 cm and 120-5 gram, respectively. Once every day, fish fed commercial fish meal. They were acclimated in 40 L aquariums (60 x 40 x 50 cm) with continuous aeration and 16- and 8-hours photoperiod for 12 days prior to the commencement of the experiment. For six weeks, two groups of fishes were treated to doses of 250 and 500 mg/l. Samples were taken six weeks after the ZnO NPs exposure. Five fish from every ZnO NPs treated group as well as the associated controls were taken out, killed, and preserved in formalin after necessary exposure times for tissue samples (liver, gills, kidney, and ovaries). The tissues samples exhibit pathological changes as a result, particularly at concentrations of 500 mg/l of Zinc oxide nanoparticles.