Molecular study of p53 supressor gene and Bcl-2 oncogene by DNA In Situ Hybridization technique in breast cancer patients in Iraq\Wasit Province

Abstract

DNA In Situ Hybridization technique was used to estimate overexpression of p53 and Bcl-2 in different histological type of Iraqi female breast cancer patients and to assess whether these biomarkers are significantly correlated with clinicopathological parameters (tumor grade , stage , age and invasiveness ) of breast carcinoma .The study included forty six patients, their ages ranging between 20-77 years with a mean age of (46.08) years, between Septembers 2012 and May 2013. The results showed that p53 overexpression frequency was (63.04) and Bcl-2 was (52.17) in patients with significant differences (p=0.001). Also the results showed asignificant differences between the expression of each of these biomarkers with tumor grade and stage (p=0.05). As well as there was significant difference between the expression of p53 and patient age (p=0.05). The results of these study supported the importance role of these genes in carcinogenesis and ability to use both genes as a markers in diagnosis.