Detection of some genetic mutations in H-Ras gene in acute and chronic leukemia

Abstract

Leukemia is one of the most prevalent forms of cancer worldwide in men, women and children with high rate of mortality. Fifty samples of blood were collected from Iraqi patients who were clinically diagnosed by the consultants of the National Center of Hematology and Medical City and 25 healthy as a control group. By direct sequencing in Macrogen company for PCR products ; nineteen mutations were detected in codon 61 of H-ras gene in acute leukemia patients , 11 of them (57.89%) were substitutions and 8 (42.1%) were deletions. Moreover, the adenine found to involve in 14 (73.68%) of 19 mutations in AML detected in this study. Most of A abnormalities involve either trans version with C and T (7 transversions) or deletion of A. H-ras mutations were also detected in chronic leukemia patients, the H- ras mutations was 12 mutations including 7 (58.33%) deletions and 5 substitutions (41.66%) .