Isolation, screening and characterization of hydrocarbon-degrading bacteria isolated from oil contaminated soil in Wasit province / Iraq

Abstract

Eleven soil samples were collected from one of the oil sites in Wasit Governorate.65 bacterial isolates were isolated from soil samples by using BHM with 1% crude oil as carbon source. In primary and secondary screening (10) isolates of bacteria can remediate crude oil. Among the ten isolates, bacterial isolate named HD2 was most effective in bioremediation process. The results of biomass, optical density, and percentage of hydrocarbon remediation of HD2 were (0.63, 1.080 and 67 %) respectively. The results of morphological and biochemical tests showed that the bacterial isolate named HD2 belongs to the species Pseudomonas putida.